Abstract:
A study has been conducted to determine the levels of serum IgA in rats (Rattus
novegicus) following intranasal exposure of the live attenuated Pasteurella multocida
B2. The importance of the study is to develop potential vaccine for Hemorrhagic
Septicaemia using the induction of mucosal immunity to secrete IgA via intranasal
route with live attenuated Pasteurella multocida B2. Enzyme-Linked Immunosorbent
Assay (ELISA) was used to determine the IgA levels in the serum of rats after the first
(Day 7) and second (Day 21) exposure. Attenuation of bacteria was achieved by
culturing the bacteria in descending percentage of blood agar starting from 5%, 4%,
3%, 2%, 1 % and lastly 0% in growth media at one-day interval for three days. Results
showed that there were no significant differences (p > 0.05) among control (p =
0.054) and treated (p = 0.056) groups.
