Determination of immunoglobulin a (IgA) in rats exposed to live attenuated Pasteurella multocida B2

Abstract

A study has been conducted to determine the levels of serum IgA in rats (Rattus novegicus) following intranasal exposure of the live attenuated Pasteurella multocida B2. The importance of the study is to develop potential vaccine for Hemorrhagic Septicaemia using the induction of mucosal immunity to secrete IgA via intranasal route with live attenuated Pasteurella multocida B2. Enzyme-Linked Immunosorbent Assay (ELISA) was used to determine the IgA levels in the serum of rats after the first (Day 7) and second (Day 21) exposure. Attenuation of bacteria was achieved by culturing the bacteria in descending percentage of blood agar starting from 5%, 4%, 3%, 2%, 1 % and lastly 0% in growth media at one-day interval for three days. Results showed that there were no significant differences (p > 0.05) among control (p = 0.054) and treated (p = 0.056) groups.