Abstract:
An efficient method for electrically introducing pCAMBIA 1304 circular plasmid into
Chlorella cells was developed. This method is useful for molecular genetic studies
and commercial applications where the genes of interest responsible for the
production of a valuable nutrient are electrically introduced into the recombinant
Chlorella sp. The pCAMBIA 1304 circular plasmid carrying the hygromycin
phosphotransferase (hpt) selectable marker, green fluorescent protein (gfp) and �
glucuronidase (gus) reporter genes regulated by CaMV 35S promoter was used to
transform the Chlorella cells. The pCAMBIA 1304 circular plasmid was successfully
extracted from E. coli and verified by PCR technique with three sets of primers
combination.
