Electroporation of Chlorella sp. with pCAMBIA 1304 circular construct

Abstract

An efficient method for electrically introducing pCAMBIA 1304 circular plasmid into Chlorella cells was developed. This method is useful for molecular genetic studies and commercial applications where the genes of interest responsible for the production of a valuable nutrient are electrically introduced into the recombinant Chlorella sp. The pCAMBIA 1304 circular plasmid carrying the hygromycin phosphotransferase (hpt) selectable marker, green fluorescent protein (gfp) and �­ glucuronidase (gus) reporter genes regulated by CaMV 35S promoter was used to transform the Chlorella cells. The pCAMBIA 1304 circular plasmid was successfully extracted from E. coli and verified by PCR technique with three sets of primers combination.