Screening of bioactive compounds from bacteria associated with marine and aquatic organisms

Abstract

Bacteria are well known associated with marine and aquatic organisms that produced valuable compounds that can be used worldwide. The aim of this study is to screen the bioactive compounds from bacteria associated with marine and aquatic organisms. This project was conducted based on air-membrane surface cultivation (AMS) and Planctonic suspension shake flask culture (PSS) method to produce bioactive compounds. Cell free supernatant (CFS) was obtained by using sterile filter syringe and impregnated onto 6mm Whatman paper disc. Disc Diffusion Test (DDT) carried out, with all discs impregnated with CFS. Test strain for DDT was Micrococcus luteus. There were 196 isolates cultivated, 32 from sponges and 164 from freshwater fishes. The results showed that 14 out of 196 from isolates cultivated, were screen with the inhibition zone ranging from 7mm to 15mm, with all 14 from bacteria isolates from freshwater fishes and no activity revealed from bacteria associated with sponges. Results indicated that media selective and surrounding environment might influence the production of bioactive compounds. As conclusion, AMS cultivation method seems to be the best technique for producing bioactive compounds compared to PSS flask culture. Bacteria associated with freshwater fish seemed to produce useful compounds.