Screening of bioactive compounds from bacteria associated with marine and aquatic organism

Abstract

Searching for the bioactive compounds from the natural sources are actively conducted by many researches worldwide in order to produce antimicrobial, antitumor and any others beneficial compounds. Therefore, the aim of this study is to screen antimicrobial activity from bacteria associated with marine and aquatic organisms. The screening of the compounds was conducted by using two types of cultivation methods; "Air Membrane Surface" and Planktonic Suspension Shake Flask" culture. The Cell Free Supernatant from these two types of cultivation methods was filtered using filter syringe and impregnated onto the discs to undergo disc diffusion test. There were 196 bacteria isolates have been cultivated, 58 of them were collected from sponges and 138 were from freshwater fish. The results showed that 14 bacteria isolates from freshwater fish samples using AMS culture were screened with inhibition zone vary from 6 to 16 diameters. Based on the results, factors might involve in the production of bioactive compounds was the growth condition, especially the composition of the culture medium. It was also proven that the AMS cultivation method seems the best approach to produce bioactive compounds compared to Planktonic Suspension Shake Flask.