Abstract:
Recently, lipase enzyme has received overwhelming attention from many researchers due
to its ability to catalyze the hydrolysis of triacylglycerol into free fatty acids and glycerol.
Besides, lipase also significantly transforms lipase's residues into useful products for
industrial usage. However, there are few studies reported on marine fungi which can
produce lipase enzyme. In this study, the ability to produce lipase by Humicola
alopallonella previously isolated from Avicennia alba was investigated. The fungal
culture was grown in tryptone broth and extracellular lipase was assayed for its activity
using cupric acetate-pyridine colometric method. In the optimization of the assay, the
effect of amount of enzyme was investigated where 1.0 ml showed the highest hydrolytic
activity on olive oil as substrate, compared to 0.2 ml and 2.0 ml.
