Abstract:
Genotoxic effect of Acanthamoeba lysates was studied on human cervical cancer cell
line. Lysates were obtained from Acanthamoeba polyphaga which are freshwater
isolate and Acanthamoeba (HKL isolate) which is a clinical isolation from corneal
scrapings of keratitis patient. These lysates were treated on HeLa cells by using MTT
assay. The mitochondrial dehydrogenases in living cells reduced the MTT substances
to a blue-magenta colored formazan crystals. The number of intact alive cells was
directly proportional to the amount of crystal formed. Results obtained from the study
indicated that the concentration of lysates that inhibited 50% (IC50) of the cancer cells
population for Acanthamoeba polyphaga and Acanthamoeba (HKL isolate) lysates are
85.0 µg/mL and 3.0 µg/mL, respectively while the IC25 values are 42.5 µg/mL and 1.5
µg/mL, respectively. The genotoxic effect of these lysates on HeLa cells by their IC50
and IC25 values were studied by using Comet assay. From the DNA damage observed,
Acanthamoeba (HKL isolate) lysate showed severe genotoxic effect compared to the
lysate of Acanthamoeba polyphaga.
