Abstract:
Microbes are the major sources of lipases where of late, marine and aquatic microbes
have become alternative sources to their terrestrial counterparts. In this study, the
ability to produce lipase by Trichoderma sp. which was previously isolated from
mangroves was investigated. The fungal culture was grown in tryptone broth and
extracellular lipase was assayed for its activity using the cupric acetate-pyridine
colorimetric method. In the optimization of the assay, the effect of amount of enzyme
was investigated where 1.0 mg showed the highest hydrolytic activity on olive oil as
substrate, compared to 0.2 mg and 2.0 mg. In order to optimize the production of
lipase, three growth parameters were studied: incubation time (24, 48 and 72 hours)..
