Abstract:
Some of the pathogenic bacteria in freshwater fish can cause disease in human hence
the identification of bacteria from fish will help to prevent contamination in human.
Bacteria from fish also could be a potential indicator for environmental pollution and
play important roles in the maintenance of water quality. The aims of this study are to
isolate the bacteria from freshwater fish, Rasbora notura and to identify and
characterize the bacteria isolates. Gram-staining and seven biochemical tests were
carried out in these study including oxidase, catalase, MR, VP, starch hydrolysis, TSI
and SIM. 91 bacteria isolates were. successfully isolated from R. notura but only 14
different species of bacteria isolates have been identified up to genus and species level
which were Megasphaera sp., Veillonella sp., Aeromonas sp., Bacillus sp., Neisseria
sp., Staphylococcus sp., Micrococcus sp., Pseudomonas sp., Corynebacterium
kutsceri, Corynebacterium xerosis, Enterbacter aerogenes, Enterbacter intermedius,
Klebsiella pneumoniae and Serratia liquefaciens. Gram-negative bacteria were
dominant in R. notura and in agreement with previous study which almost 90 percent
of Gram-negative bacteria were dominant in freshwater animals. The dominance of
Megasphaera sp. and Klebsiella pneumoniae in this study indicates that fecal
contamination was occurring in Paya Sungai Udang.
