Abstract:
Weedy rice (Oryza sativaf spontanea) is taxonomically classified as the same species as
cultivar rice (Oryza sativa) but has characterized by seeds strong difference in seeds
shattering and dormancy. Weedy rice has serious effect on rice population by causing
yield reduction and affects the quality of rice grains. Thus, this study was aimed to
develop RAPD marker, which could be used to differentiate cultivar and weedy rice. All
together, nine different RAPD primers were used to screen the genomic DNA extracted
from cultivar rice (CR) and three different biotypes of weedy rice (WRl, WR2 and
WR3). Screening results revealed that two RAPD primers designated A-04 and A-09
produced specific bands that have the potential to be used as marker to discriminate
between rice and weedy rice biotypes.