Screening of cultivar and weedy rice with RAPD primer.

Abstract

Weedy rice (Oryza sativaf spontanea) is taxonomically classified as the same species as cultivar rice (Oryza sativa) but has characterized by seeds strong difference in seeds shattering and dormancy. Weedy rice has serious effect on rice population by causing yield reduction and affects the quality of rice grains. Thus, this study was aimed to develop RAPD marker, which could be used to differentiate cultivar and weedy rice. All together, nine different RAPD primers were used to screen the genomic DNA extracted from cultivar rice (CR) and three different biotypes of weedy rice (WRl, WR2 and WR3). Screening results revealed that two RAPD primers designated A-04 and A-09 produced specific bands that have the potential to be used as marker to discriminate between rice and weedy rice biotypes.