Effects serum IgA in white rats vaccinated with killed Pasteurella multocida B2 Orally.

Abstract

Pasteurella multocida B2 is the main cause of Haemorragic septicaemia (HS), a cronic disease that infects catties and buffoles in the region of Asia. Therefore,a study was done to induce the production of Immunoglobulin a (IgA) as to increase the protection against this disease. Thus, this study was carried out to determine the concentration and level of lgA in serum followed by exposure to formalin killed Pasteurella multocida B2. In the study, 36 clinically healthy female Albino Winstar rats were divided into four groups according to the dosage that will be exposed; Group 1 (10% vaccination dosage), Group 2 (30% vaccination dosage), Group 3 (50% vaccination dosage) and Group 4 (negative control). There were two oral vaccination exposures done in week 1 and week 3 in the form of food prepared. Enzyme-linked immunoabsorbent assay (ELISA) was subjected to the serum samples collected once every week from each rats from all the groups respectively to determine the level of lgA. As a result, significant difference (p<0.05) of lgA were obtained among all the groups in the study.