Abstract:
The herbicides resistant weeds become a major threat in Malaysia's plantation.
Eleusine indica (L.) Gaertn appeared as resistance to glyphosate in 1997. Hence, the
development of C319 allele specific marker will help in controlling the weed in the
effective ways. Genomic DNA extracted from susceptible and different level of
resistances of goosegrass was used in this study. EPSPS allele specific clones were
generated by cloning of the PCR fragments. EPSPS-C319 marker was optimized and
used in screening of different resistant level of goosegrass with the EMC-F/ES2-R
primer combination. The PCR optimization showed that the optimum condition was at
35 cycles and annealing temperature of 61 °C. Susceptible biotypes showed the
presence of C allele in all ten samples. The resistant of 6-fold and 4-fold also showed
the existence of C allele at specific band of 628 bp while no band produced in resistant
8-fold. Only C319 allele specific marker was successfully developed in this study thus
further research on other allele specific marker could be carried out in the future.
