Abstract:
Identification of bacteria from polluted areas including freshwater environment are
important especially for health development to mankind. To date, bacteria are used for
bio-indicator and proven to be the fastest method of identifying polluted area. The
aims of this study are to isolate, to identify and to characterize bacteria from
freshwater fish, 0. anomalura. A hundred of bacteria isolates were cultured on
MacConkey and Nutrient agar. Biochemical tests were used to identify bacteria
isolates at genus and species level. The genus and species that had been identified
were Serratia fonticola, Aerornonas sp., Aeromonas salmonicida, Citrobacter
freundii, Klebsiella pneumoniae, Serratia sp., Neisseria flavescens, Neisseria sp.,
Serratia liquefaciens, Enterobacter aerogenes, Staphylococcus sp., Corynebacterium
xerosis, Citrobacter diversus, Aerornonas veronii, Enterobacter interrnedius,
Pseudomonas sp. and Corynebacterium kutsceri. The most abundant bacteria isolated
were from Gram-negative bacteria which were from Neisseria sp., 20% followed by
Staphylococcus sp., 13 %. The abundance of Staphylococcus sp. in Paya Sungai
Udang indicated that the area was contaminated with feacal. 81 % of the total numbers
of bacteria isolates were Gram-negative bacteria. The finding of this study is in
agreement with previous study indicated that Gram-negative bacteria dominated the
freshwater fish.