Identification and characterization of bacteria from freshwater fish, Oxygaster anomalura

Abstract

Identification of bacteria from polluted areas including freshwater environment are important especially for health development to mankind. To date, bacteria are used for bio-indicator and proven to be the fastest method of identifying polluted area. The aims of this study are to isolate, to identify and to characterize bacteria from freshwater fish, 0. anomalura. A hundred of bacteria isolates were cultured on MacConkey and Nutrient agar. Biochemical tests were used to identify bacteria isolates at genus and species level. The genus and species that had been identified were Serratia fonticola, Aerornonas sp., Aeromonas salmonicida, Citrobacter freundii, Klebsiella pneumoniae, Serratia sp., Neisseria flavescens, Neisseria sp., Serratia liquefaciens, Enterobacter aerogenes, Staphylococcus sp., Corynebacterium xerosis, Citrobacter diversus, Aerornonas veronii, Enterobacter interrnedius, Pseudomonas sp. and Corynebacterium kutsceri. The most abundant bacteria isolated were from Gram-negative bacteria which were from Neisseria sp., 20% followed by Staphylococcus sp., 13 %. The abundance of Staphylococcus sp. in Paya Sungai Udang indicated that the area was contaminated with feacal. 81 % of the total numbers of bacteria isolates were Gram-negative bacteria. The finding of this study is in agreement with previous study indicated that Gram-negative bacteria dominated the freshwater fish.