Abstract:
Pasteurella multocida is a coccobacillus bacterium that has been recognized as an
important animal pathogen and this type of bacteria always exists in the respiratory
tract of different animals. Thus, this study was conducted to determine the effects
oral vaccination of killed Pasteurella multocida B:2 on Bronchus-associated
lymphoid tissue (BAL T). This study involved with forty-two clinically healthy rats
(Rattus novegicus) from type Sprague- Dawley that were randomly allocated into
two groups. Each group contains 21 rats for the 7 weeks experiment duration. Group
1 was the control untreated group while Group 2 was the treated group. The treated
groups were exposed twice with oral vaccination with two week interval and every
exposure consists 15g vaccinated pellet with killed Pasteurella multocida B:2 for
each rat. Then, every week on a designated day, three white rats from the each group
were slaughtered to collect the rat's lung samples throughout project for seven
weeks. After that, histological works was done to all lung samples to observe
bronchus-associated lymphoid tissue (BAL T). The results of the study using oral
vaccination on the area of BAL T and the number of lymphocytes show a very
positive response having (p< 0.01) between the control and the treatment group. The
data analysis was conducted using the ANOV A Two-Factor with Replication. The
response between the weeks also shows a significant difference having (p< 0.05) in
both area ofBALT and the number of lymphocytes. Results obtained proved that the
BAL T response well and the oral vaccination manage to induce immunity towards
the Pasteurella multocida B:2 in rats.
