Screening and characterization of bacteria producing antibacterial from marsh soil

Abstract

Soil microbial communities have been proposed as antibacterial agents are widely used to control diseases. However, there is often too little information reliably about microorganisms from soil marshes for this purpose. The objective of this study is to screen, characterize and identify the bacteria that producing antibacterial agents from marsh soil. This paper presents the results of field surveys at several study marsh soil within the Universiti Malaysia Terengganu Swamp and Setiu Wetland. The methods in order to detect antibacterial resistant produce by isolated bacteria were using disc diffusion method. Gram staining and six biochemical tests were carried out to characterize these bacteria including MR, oxidase, catalase, spore stain, starch hydrolysis, acid fast stain and strict anaerobes. These isolated bacteria were identified through BBL crystal kit and Bergey's Manual of Determinative Bacteriology. As a result, 11 isolates were isolated from samples that have antimicrobial activity to provide antibiotic. It has been found that isolates are effective against Pseudomonas sp., Staphylococcus aureus and Escherichia coli. This study also indicated that the escalating misuse of antibacterial will be solved.