Identification and characterisation of bacteria from freshwater fish (Rasbora banknensis)

Abstract

The knowledge of isolation and identification of bacteria from aquatic animal and aquatic environment is expanding at a rapid rate. Recent study indicated that there has been great interest in studying the occurrences and distribution of the various bacterial pollution indicators in water and the associated environment Rasbora bankanensis is fresh water fish that caught at the Paya Sungai Udang, Kuala Berang, Hulu Terengganu. Paya Sungai Udang is one of the polluted rivers at this area. The aims of this study are to identify and characterize of bacteria isolated from R. bankanensis. The bacteria were isolated from two parts of the fish which were external and internal of fish body. The internal part of fish included gill and intestine. In this study, a few methods were used including isolation of bacteria, Gram-staining and micromorphology method and biochemical tests. As the results, about 103 bacteria were managed to isolate and all the bacteria were successfully identified. Result indicated that 12 genus bacteria isolated which were Klebsiella spp. was the most abundant bacteria that are 28%. It was because the Klebsiella spp. was from the family Enterobacteriaceae that have higher percentages among other bacteria. The second highest bacteria that manage to identify was Enterobacter spp. which was 26% and followed by Pseudomonas spp. 14%, Staphylococcus spp. 10%, Yersinia spp. 5%, Serratia spp. 4%, Bacillus spp. 4%, Aeromonas 3%, Moraxella spp. 2%, Escherichia spp. 2%, Shigella spp. l % and Citrobacter spp. 1 %. Result indicated that Klebsiella spp. were abundance in R. bankanensis. Results obtained with high population of Klebsiella spp. as a result of polluted area of Paya Sungai Udang with faecal contaminations.