Isolation of Pasteurella multocida from local and imported goats in commercial farm in Terengganu

Abstract

Pasteurella multocida that was well known as a major causes of pasteurellosis especially in farm small ruminant such as goats and lambs. Haemorrhagic septicaemia and pneumonic pasteurellosis which are the two major diseases that cause by P. multocida. Nowadays, this Pasteurellacea family has become one of the enemies to the farmer for this century. The purpose of this research is to determine and compare the prevalence rate of P. multocida from nasal of the pre-weaning, weaning and adult of local and imported goats before and during monsoon season. In this research, one hundred and twenty right nasal swab samples were collected from goats at Ladang Rakyat Terengganu, which are twenty samples from each group; pre-weaning, weaning and adult of local and imported goats. This research was replicate in six times. 5% sheep blood agar was used for growth of these bacteria. Gram staining and biochemical test such as oxidase, catalase, indole and triple sugar iron (TSI) were used for bacteria identification. For further confirmation of bacteria, API 20E were going on. Results from this research, we found that the P. multocida were not isolated before monsoon season. However, P. multocida were successfully isolated for during monsoon season. Imported weaning groups recorded as a highest prevalence rate of P. multocida. Decreased in the immunity level and stress after separating from their mother may act as causes why P. multocida can isolated more higher in weaning group compared to the other group. On the other hand, the overall percentage of P. multocida isolation is still lowest, which is only 1.53%. This probably due to the vaccination program at Ladang Rakyat Terengganu was very efficient.