Screening and characterization of bactera producing antibacterial from clay soil

Abstract

Microorganisms that have a capability to produce antimicrobial properties are widely distributed in nature. Discovery of microorganisms especially bacteria from soil that have a potential in producing antibiotic will become a contribution for a better human life. The aims of this study were to isolate bacteria producing antibiotic from clay soil, characterize and identify the bacteria through their morphology, colony and biochemical activities, and to detect antibiotic resistant produce by isolated bacteria with commercial antibiotic towards the testing bacteria. The methods that used in this study including soil sampling a paddy field in Maras, Terengganu , growing and isolating of bacteria, and also screening and characterization of isolated bacteria through disk diffusion, plugs and antagonist method followed by identification of bacteria using BBL Crystal ID System. All the bacteria were characterized macroscopically and microscopically. An amount of 10 different types of bacteria were detected showing an antibiotic potential and 5 of them were Brevibacillus brevis, Bacillus cereus, Corynebacterium aquaticum, Streptococcus uberis and Agrobacterium tumefaciens Previous study stated that Genus Bacillus was proven have an antibiotic potential and the others still have not well known. All the bacteria may be useful in future research in order to gain avail product from them.