Effects serum IGG in white rats vaccinated with killed Pasteurella multocida B:2

Abstract

Pasteurella multocida B:2, a Gram-negative pathogenic bacteria has been recognized as the causative agent of Haemorrhagic septicaemia (HS), a disease that infects cattle and wates buffaloes in the region of Asia. One of the best method to increase the protection against HS is by inducing the production of Immunoglobulin (IgG). Therefore, a study was conducted to determine the concentration of IgG in the serum of white rats following oral exposure of killed Pasteurella multocida B2. Thirty-six clinically healthy female Albino Winstar white rats were divided into four groups. White rats in Group 1, Group 2 and Group 3 is subjected to double oral exposure to 10%, 30% and 50% of killed Pasteurella multocida B:2 in food pellet respectively. White rats in the Group 4 were the control group which was left unexposed. Serum samples were collected once every week from each group of rats. Enzyme-linked immunosorbent assay (ELISA) was used to measure the lgG titer in the blood serum. As the results, oral vaccination of Pasteurella multocida B:2 has induced an immune response to the rats in Group 1 and Group 2 significantly (p<0.05) and Group 3 insignificantly (p>0.05). Neutralization was performed by lgG after second exposure due to lgG titer which was still on the rise when second exposure was made. lgG is long standing and is crucial to obtain satisfactory protection against most infection. It is concluded that oral vaccination has effect on serum IgG after double exposure of Pasteurella multocida B:2 to white rats. Further research are recommended to prolong the interval between the two exposures to measure the maximum peak of lgG after oral vaccination with killed Pasteurella multocida B:2