Abstract:
A Cryptocoryne minima is an aquatic plant from family Aracea. In vitro of C. minima
were successful proliferated in B5 medium. The objective of this study is to determine
the best medium for cultures and the effect of cytokinins on growth of Cryptocoryne
minima. The explants were divided and cultured into treatment media containing
Kinetin, 6-benzylaminopurine (BAP), Zeatine or 6-(y,y- dimethylallyamino) purine
(2iP) at different concentration. The formation and changed were observed and
recorded weekly. All explants that receiving various type and concentration of
hormone showing differences in shoot and petiole length, shoot width, multiplication
rate (number of new shoot per explants), and development of roots. The size of C.
minima in liquid was bigger than solid media. A comparison of cytokinins activity
showed that the best shoots proliferation was obtained from cultures treated with 6-benzylaminopurine
(BAP).There was a greater development of new roots on medium
supplemented with no hormone but suppressed new shoots formation.
