Abstract:
Antisense palmitoyl-ACP thioesterase will suppress the production of palmitic acid in
plant fatty acid biosynthesis and could reduce the level of palmitate in plant. Thus, the
nutritive value and the industrial uses of vegetable oils can be increased. The 35S-AP
fragment contains antisense palmitoyl-ACP thioesterase cDNA was extracted then
amplified by PCR using PTEAP-Fl and PTEAP-R2 primers. The DNA fragment
(-1600 bp) was successfully recovered from the gel slide and was cloned into
pCAMBIA 1301 vector. The putative recombinant clones were screening with colony
PCR to confirm the presence of DNA insert, with three different primer combinations:
PTEAP-R2/PTEVR2; PTEAP-Fl/PTE-VFl and PTEAP-Fl/PTEAP-R2.
