Construction of pASPTE 1301 vector for genetic manipulation of microalgae.

Abstract

Antisense palmitoyl-ACP thioesterase will suppress the production of palmitic acid in plant fatty acid biosynthesis and could reduce the level of palmitate in plant. Thus, the nutritive value and the industrial uses of vegetable oils can be increased. The 35S-AP fragment contains antisense palmitoyl-ACP thioesterase cDNA was extracted then amplified by PCR using PTEAP-Fl and PTEAP-R2 primers. The DNA fragment (-1600 bp) was successfully recovered from the gel slide and was cloned into pCAMBIA 1301 vector. The putative recombinant clones were screening with colony PCR to confirm the presence of DNA insert, with three different primer combinations: PTEAP-R2/PTEVR2; PTEAP-Fl/PTE-VFl and PTEAP-Fl/PTEAP-R2.