Optimal condition of marine actinomycete and its antimicrobial activities

Abstract

Marine actinomycete isolated from Bidong Island seawater was screened for antimicrobial activities against a panel of selected bacteria and fungi. Positive results against yeast Saccharomyces cerevisiae, gram-positive of Streptococcus agalactiae, Staphylococcus aureus and Bacillus subtillis and gram-negative of Klebsiella pneumonia, Pasteurella multocida except Aeromonas hydrophila and Escherichia coli were noted. Determination of optimal temperature and salinity for the growth of marine actinomycete cultured artificially in the laboratory was done and followed by antimicrobial susceptibility testing on target S. agalactiae. The best group of marine actinomycete in terms of secondary metabolites production was the one cultured at 27°C with O ppt salinity. The optimized growth however was from the culture condition at 37°C with O ppt which suggested the possibility of terrestrial origin. Correlation is significant, in which salinity and temperature showed moderate and high correlation (r = -0.641 and 0.734) respectively with marked relationship towards the dependant values of marine actinomycete growth. [The growth of marine actinomycete affected by temperature and salinity using this fundamental testing of measuring diameter colonies was significant (p<0.01)]. The results showed the ability of marine actinomycete to produce antimicrobial activities and its potential as an antimicrobial agent from marine resource. The people who involve in antimicrobial research should undertake the findings forward especially in determining the actual/definite strength of this species against different types of target microbes.