Abstract:
Isolation of bacteria from different part of sea cucumber, Holothuria edulis, body that
was collected from Bidong Island was carried out. From the study that has been made,
there were 6 different colonies of bacteria was isolated from every part of the H.edulis
body (skin, surface of the body, internal mucus and cloacae opening). From experiment
in the production of polysaccharides, bacteria identified as Acinetobacter calcoaceticus
was believe to produce high yield of polysaccharides and was chosen for further test.
This bacterium is of the type gram-negative. This identification achieve with the
combination result from biochemical test and also by using RapID™ ONE System
(REMEL, USA). Average of crude polysaccharide which has been produced by
Acinetobacter calcoaceticus was 235.9mg / L while average of acidic polysaccharide
produced was 165.1mg / L. From Paper Chromatography (PC) and High Performance
Liquid Chromatography (HPLC) analysis shows that polysaccharides produced by
Acinetobacter calcoaceticus contain basic sugar compositions which were galactose,
glucose, lactose and raffinose.
