Isolaton, identification and purificaion of polysaccharides derived from bacterium associated with sea cucumber, Holothuria edulis

Abstract

Isolation of bacteria from different part of sea cucumber, Holothuria edulis, body that was collected from Bidong Island was carried out. From the study that has been made, there were 6 different colonies of bacteria was isolated from every part of the H.edulis body (skin, surface of the body, internal mucus and cloacae opening). From experiment in the production of polysaccharides, bacteria identified as Acinetobacter calcoaceticus was believe to produce high yield of polysaccharides and was chosen for further test. This bacterium is of the type gram-negative. This identification achieve with the combination result from biochemical test and also by using RapID™ ONE System (REMEL, USA). Average of crude polysaccharide which has been produced by Acinetobacter calcoaceticus was 235.9mg / L while average of acidic polysaccharide produced was 165.1mg / L. From Paper Chromatography (PC) and High Performance Liquid Chromatography (HPLC) analysis shows that polysaccharides produced by Acinetobacter calcoaceticus contain basic sugar compositions which were galactose, glucose, lactose and raffinose.