Abstract:
This study was conducted to determine the production of chitosan from marine crab,
Scylla serrata. Crab shell need to undergo of dimineralization (DM), deproteinizition
(DP), and deacetylation (DA) process in order to produced chitosan. Purpose of this
study was to produce chitin and chitosan from the crab shell, with two different
method been compared (reversing method and traditional method. Physicochemical
and functional properties effect of sample due to the changes in modification or
reversing method, where demineralization initiate the method but not in
deproteinization process which commonly practiced in traditional method. The
chitosan yield from modification method (DMPA) is higher with 53.7% compared
with 53.0% from traditional method (DPMA). The result from physicochemical
analysis (moisture and ash content) shown that moisture content in traditional method
is lower than modification with l .3 % to 1.7% respectively. The results from both
samples are acceptable. For ash content, the percentage of modification method is
lower than traditional method with 78.7% and 85.8% respectively. Chromatography
paper and HPLC analysis showed glucose and glucosamine present in sample of
chitin and chitosan.
