Production of chiosan from marine crab, Scylla serrata

Abstract

This study was conducted to determine the production of chitosan from marine crab, Scylla serrata. Crab shell need to undergo of dimineralization (DM), deproteinizition (DP), and deacetylation (DA) process in order to produced chitosan. Purpose of this study was to produce chitin and chitosan from the crab shell, with two different method been compared (reversing method and traditional method. Physicochemical and functional properties effect of sample due to the changes in modification or reversing method, where demineralization initiate the method but not in deproteinization process which commonly practiced in traditional method. The chitosan yield from modification method (DMPA) is higher with 53.7% compared with 53.0% from traditional method (DPMA). The result from physicochemical analysis (moisture and ash content) shown that moisture content in traditional method is lower than modification with l .3 % to 1.7% respectively. The results from both samples are acceptable. For ash content, the percentage of modification method is lower than traditional method with 78.7% and 85.8% respectively. Chromatography paper and HPLC analysis showed glucose and glucosamine present in sample of chitin and chitosan.