Abstract:
The marine sponge, Xestospongia sp. was used in study of the production and
purification of polysaccharides from marine bacterium isolated from it. The bacterium
that isolated from the sponges had been carried out in the investigation of their culture,
morphological then identify the bacterium. This study also identified the chemical
composition of the polysaccharides that produced by isolated bacterium isolated from
Xestospongia sp. RapID TM ONE Plus System (REM EL, USA) was used in identification
of isolated bacterium then namely as Enterobacter cloacae after the biochemical test
were carried out. The entire biochemical test characterized the characteristics of the
bacterium. The average yield of the crude polysaccharide isolated from Enterobacter
cloacae was 624.5 mg per I L of the medium and the average yield of acidic
polysaccharide was 241.0 mg per I L medium. Elution profile of the acidic
polysaccharide on DEAE-cellulose columns shows the highest peak occurred at 0.4 M
NaCl while there are no peak occurs at O M NaCl. The determination of chemical
composition in the bacterium was performed by using paper chromatography (PC) and
High Performance Liquid Chromatography (HPLC).For PC same sugar composition
whether in crude or acidic polysaccharide was presence which is arabinose, galactose and
maltose Results for HPLC chromatogram of hydrolyzed acidic polysaccharide with 2 M
HCI containing glucose, arabinose, galactose and maltose. However, the result for crude
polysaccharide gave only glucose, arabinose and galactose.
