Production and purification of polysaccharide from marine bacterium isolated from marine sponge, Xestospongia sp.

Abstract

The marine sponge, Xestospongia sp. was used in study of the production and purification of polysaccharides from marine bacterium isolated from it. The bacterium that isolated from the sponges had been carried out in the investigation of their culture, morphological then identify the bacterium. This study also identified the chemical composition of the polysaccharides that produced by isolated bacterium isolated from Xestospongia sp. RapID TM ONE Plus System (REM EL, USA) was used in identification of isolated bacterium then namely as Enterobacter cloacae after the biochemical test were carried out. The entire biochemical test characterized the characteristics of the bacterium. The average yield of the crude polysaccharide isolated from Enterobacter cloacae was 624.5 mg per I L of the medium and the average yield of acidic polysaccharide was 241.0 mg per I L medium. Elution profile of the acidic polysaccharide on DEAE-cellulose columns shows the highest peak occurred at 0.4 M NaCl while there are no peak occurs at O M NaCl. The determination of chemical composition in the bacterium was performed by using paper chromatography (PC) and High Performance Liquid Chromatography (HPLC).For PC same sugar composition whether in crude or acidic polysaccharide was presence which is arabinose, galactose and maltose Results for HPLC chromatogram of hydrolyzed acidic polysaccharide with 2 M HCI containing glucose, arabinose, galactose and maltose. However, the result for crude polysaccharide gave only glucose, arabinose and galactose.