Abstract:
The sea cucumber, Holothuria atra was used in this study in order to isolate and identify
the associated bacterium. A bacterium was selected from 5 different colonies (G 1, G2,
G3, G4 and G5) of bacteria obtained from the media cultured. The isolation and
identification were done to produce and purify polysaccharide from that bacterium. This
study also analyses the chemical composition of polysaccharide produced by the selected
bacterium.The selected bacterium isolated from sea cucumber was identified as Gram
Negative bacterium, Sphingomonas paucimobilis by using RaplD
TM
NF Plus system. The
identification was obtained by combining of result from the biochemical characteristics.
The yield of crude polysaccharide produced was 608.2 milligram (mg) per 1 liter (L)
while the acidic polysaccharide was 231.6 mg per 1 L. Most of the purified acidic
polysaccharide produced was from 0.4M to 0.6M concentration of NaCl buffer. The
analyses of polysaccharide were carried out using Paper Chromatography (PC) and High
Performance Liquid Chromatography (HPLC). The sugar presence for PC analysis was
maltose, galactose and rhamnose for crude and purified acidic polysaccharides. The
HPLC shows the same sugar presence as PC analyses except for crude polysaccharide
with the absence of maltose sugar.
