Gene expression profiling of Clarias batrachus FED with omega-3 enriched pellet and non omega-3 enriched pellet

Abstract

A study was done to determine the gene expression profiling in Cf arias batrachus fed with omega-3 enriched and non omega-3 enriched pellet. This study involves several steps beginning with sample collection, followed by RNA extraction and purification, RNA quantification, gene expression profiling using (DD) RT-PCR technique, analysis of gene expression profile and isolation of expressed genes. A total of 5 samples of Asian catfish, Clarias batrachus fed with Omega-3 enriched pellet non omega-3 enriched pellet were collected. The results of the experiment showed that there was a difference in the gene expression profiling between C.batrachus fed with omega-3 enriched and non omega-3 enriched pellet when amplified using Arbitrary Primer ACP2, ACP5, ACP7, ACP 11, ACP 17 and ACP20. Range of DNA fragments obtained from the experiment were within 1 OObp to 1300bp. Overall observation found that several genes have been down-regulated in C.batrachus fed with omega-3 enriched pellet as it contained less DNA fragments compared to C.batrachus fed with non omega-3 enriched pellet.