Induction of triploidy in Patin bunga, Pangasius sutchi by heat shock treatment

Abstract

The use of triploidy induction, in aquaculture has attracted considerable attention. Triploidy induction is an effective way to achieve sterility in fish. Sterility confers a potential additional advantage, as the energy invested in reproduction can be diverted to somatic growth. Triploidy induction is a process whereby an entire chromosome set is added to a fertilized egg. The objectives of the present study were to determine the possibility of inducing triploid Pangasius sutchi using heat temperature shocks and to determine the success of triploid by erythrocytes measurement. Heat shock treatments were applied to the eggs 2 minutes after fertilization. Fertilized eggs were subjected to preselect temperatures at 36, 38, 40 ° and 42 C at duration time 0.5, 1.0, 1.5 and 2.0 minutes. Control groups were kept separately at ambient temperature (26.5 ° C). The success of triploidy were assessed by erythrocytes measurement. Sampling to determine growth rates (total length) were also be carried out until the larvae reached 6 weeks of age. From the results, it was proven that the average size were greater than those of diploids with approximately 1.51 times larger than the diploids.