Abstract:
Citrobacter spp. is a member of the family Enterobacteriaceae. They are commonly
called a group of bacteria that inhabit the intestine tract of humans and other animals.
In fish, Citrobacter spp. infection may cause skin ulcerative lesions, distended
abdomen, pale liver, darkened spleen, watery kidney and bloody exsudate in
gastrointestinal system in fish. Bacteria of the genus Citrobacter are inhabitants of
the intestinal tract of fish and are present in sewage, surface waters and in food
contaminated with faeces material and soil. This study was conducted to isolate and
identify Citrobacter spp from tilapia hybrid fry from aquaculture fresh water ponds.
Yersinia Selective Agar, medium for the selective cultivation of Yersinia spp. were
used to obtain pure cultures of Citrobacter spp. 100 µl of bacteria cultures were
spread-platting by using a sterile hockey-stick onto medium
