Isolation and identification of green colonies Vibrio spp on TCBS Agar from cockles

Abstract

Genus and identification of bacteria species m foods and clinical specimen are important. In Malaysia, one of the most important bivalve molluscs in aquaculture is blood cockle (Anadara granosa). The consumption of bivalve molluscs is a major source of cheap animal protein from marine organisms. However, it is capable of causing food poisoning outbreak due to Vibrio spp. In the present study, the samples were bought from Wet Market located in Batu Enam, Kuala Terengganu. Conventional tests such as morphological, biochemical and physiological tests were used to identify the green colonies Vibrio spp. on TCBS agar isolated from cockles (A. granosa). Beside, by using software NTSYS pc version 2.1, the numerical taxonomy was also used to cluster the Vibrio spp. They were compared with refer database of two green colonies Vibrio species which were V parahaemolyticus and V vulnifzcus. Here the isolates were identified as V parahaemolyticus due to the similarity obtained from the numerical analysis was ranged from 76.92% to 88.89%. Isolate 6 having the lowest percentage of similarity while the highest similarity was isolate 3. The vibrios contents in blood cockles were in high amount ranged from p arahaemolyticus isolates were detected where, 8 isolates showing �-haemolysis and 3 isolates were a-haemolysis on blood agar.