The production of exopolysaccaride from marine bacteria associated with marine sponge (Aaptos spp.) using sponge medium

Abstract

The production of exoploysaccharide from the manne sponge usmg the sponge medium is one of the new research which is carried out. The objective of this study is to isolate and purified polysaccharide from gram negative bacteria associated with marine sponge Aaptos spp using both SSW medium and Aaptos spp. sponge medium. The study was also to identify isolated bacterium and the chemical analysis of the purified polysaccharide. The sponges were taken from Bidong Island and the samples were isolated from the outer layer of sponge. There are two bacteria which were isolated from this sponge which were labeled as bacteria A0-1 and bacteria Al-1. These isolated bacteria have been put for further analyses such as morphological characteristics, biochemical test and RapID ™ NF Plus and ONE System. Bacteria A0-1 and bacteria AI- I is a gram negative, straight rod and identified as Pseudomonas stutzeri and Alcaligenes faecalis. The production of crude polysaccharide from bacteria Pseudomonas stutzeri was 156 mg/I. The production of crude polysaccharide from this bacteria Alcaligenes faecalis was 240 mg/1. The analysis of sugars in this both bacteria was done by using Paper Chromatography (PC) and High Perfomance Liquid Chromatography (HPLC). From PC results, Bacteria A0-1 and Bacteria AI-1 showed the presence of maltose and glucose in SSW medium while trehalose and glucose in sponge medium.