Optimization of enzymatic hydrolysis of protein from catfish waste using response surface methodology

Abstract

This study is about optimization of enzymatic hydrolysis process of frame waste from local catfish, African catfish, Clarias gariepinus. In order to determine the optimum condition of the hydrolysis, range of parameters were chose according to related previous study. Those parameters are temperature, time, enzyme concentration and pH. Enzyme used was Alcalase. Arrangement of the level of parameter was based on a design in response surface methodology which is Central Composite design. Proximate analysis was carried out on the raw material and also fish protein hydrolysate to see the comparison of the content before and after undergoes hydrolysis process. As a result, the optimum conditions to achieve maximum degree of hydrolysis were temperature of 60°C, time of 180 min, pH of substrate at 9.5 and an enzyme concentration of 2.0%. Regression of coefficients indicates that all linear forms, which are temperature, time, EIS ratio and pH, and temperature2 were significant (P < 0.05). The product which is catfish frame waste hydrolysate has high protein content and many potential benefits such as animal feed and supplement for human can be developed through further study. Pollution problem that caused by the disposal of the waste may also be decreased.