Determination of oxidative activity and phenolic content in Hibiscus tiliaceus of matured leaves, young leaves and bark

Abstract

This study was conducted to evaluate the Antioxidant act1v1ty (AA) and phenolic compound of differences part of Hibiscus tiliaceus which was collected around mangrove area of Pantai Tok Jembal, Kuala Terengganu. The sample were H. tiliaceus matured leaves, young leaves and bark. Antioxidant activity (AA) of the methanol acetate was measured using ferric thiocyanate (FTC) mehod and 2,2-diphenyl -1-picrylhdrazyl (DPPH) method. Phenolic compound was measured by using follin-ciocalteu method. Methanol acetate were used as extracting solvent. Using FTC method, result show that matured leaves were the highest antioxidative activity but there ware no significant different (p<0.05) between all samples. However using DPPH method the result show that matured leaves were the highest antioxidative activity and there were significantly different (p<0.05) between all the samples. For phenolic content, result show matured leaves as the highest compared to all the samples and there are also significantly different (p<0.05) among the samples. Based on the result matured leaves of H. tiliaceus extract could be used as accessible source of natural antioxidant.