Effects of different cytokinin on proliferation of Cryptocoryne elliptica cultures

Abstract

Cyptocoryne elliptica is an aquatic plant which is utilised widely in aquarium industry as an ornamental plant. Due to its over-exploitation, deteriorated further by its low rate of propagation, it has become endangered. A way to increase its rate of propagation is through in vitro proliferation. Effect of cytokinin was investigated using different hormone (zeatin, benzylaminopurine, thidiazuron) with different concentration (0.2, 0.4, 0.8 mg/L) using different type of explants (basal petiole, four 2-mm consecutive parts from the apical dome of shoot apical meristem). Study showed that there was no significant difference in number of shoot formed between all treatments. However, basal petiole, section 3 and 4 of shoot apical meristem showed higher shoot regeneration rate. Morphology of shoot formed differed in colour and leaf generation rate between zeatin, benzylaminopurine and thidiazuron. Callus was also formed in only basal petiole explants in benzylaminopurine and thidiazuron. Morphology of callus also differed in colour and texture between benzylaminopurine and thidiazuron.