Abstract:
For more than two decades, acetosyringone is the phenolic compound uses in
Agrobacterium-mediated transfmmation using callus de1ived from seed as a starting
material. In the present study, the efficiency of vanillin in Agrobacterium-mediated
transformation of omega-3-desa!urase gene into rice calli was investigated. The results
showed that vanillin concentration at 200 pLM produced the highest percentage of putative
transformant on the 8J1tibiotic screening medium and P-glucuroniclase (GUS) assay
compared to control ancl400 ~-tM ofvanillin. The ability of vanillin in activating vir gene
provides an alternali ve phenolic compound for Agrobacterium-mediated gene
transformation of rice.
