Agrobacterium-mediation trannsformation of rice with omega-3- desaturase gene

Abstract

For more than two decades, acetosyringone is the phenolic compound uses in Agrobacterium-mediated transfmmation using callus de1ived from seed as a starting material. In the present study, the efficiency of vanillin in Agrobacterium-mediated transformation of omega-3-desa!urase gene into rice calli was investigated. The results showed that vanillin concentration at 200 pLM produced the highest percentage of putative transformant on the 8J1tibiotic screening medium and P-glucuroniclase (GUS) assay compared to control ancl400 ~-tM ofvanillin. The ability of vanillin in activating vir gene provides an alternali ve phenolic compound for Agrobacterium-mediated gene transformation of rice.